The centromere represents a crucial chromosomal structure necessary for proper chromosome segregation during mitosis. The research examines how centromeric chromatin forms and maintains itself, utilizing C. elegans as a model system due to its holocentric chromosome characteristics and discrete centromeric nucleosome distribution. The second research focus investigates how cellular differentiation occurs and is sustained during multicellular organism development. Using the INTACT technique to isolate nuclei from targeted cell types, researchers analyze simultaneous expression and chromatin patterns across developmental stages to understand tissue-specific chromatin organization dynamics.